Western blot blotting tank / for Southern blot / for Northern blot
WAVE Sub Blot Cleaver Scientific

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Characteristics

  • Applications:

    for Western blot, for Southern blot, for Northern blot

Description

Key Features

Ideal for wet electroblotting of proteins – Western blotting
Up to five gel blot cassettes utilised at any one time
Hinged cassettes for added convenience
Accommodates gel thicknesses from 0.25 up to 3mm

Description

The Sub Blot Mini and WAVE high intensity transfer systems combine the cooling capacity of wet submarine blotting units with the speed of semi-dry transfer systems. Both systems utilise plate electrodes to create a higher strength electric field and greater current density than conventional wire-electrode systems. This allows transfer of broad range molecular weight proteins to be achieved typically within an hour in the Sub Blot Mini, and in 1-2 hours using the WAVE Sub Blot System. Features offered by the Sub Blot Mini and WAVE are as follows:

Plate-electrodes 2cm apart in the Sub Blot Mini facilitate fast transfer of two stacked 8×8.5cm mini gels; and 4cm apart in WAVE Sub Blot system for transfer of one Maxi gel
Multi-transfer capability in the WAVE also allows transfer of 4 omniPAGE Mini and 2 omniPAGE Mini Wide gels arranged side-by-side
Two cooling options; a cool pack supplied with the Sub Blot Mini for environmentally friendly low-cost cooling; and active cooling in the Sub Blot WAVE by a detachable cooling coil connected to a recirculating chiller.
Magnetic stirring bars fit conveniently beneath the cooling coil within the WAVE tank to maximise buffer circulation and heat dissipation, while preventing ion gradient formation; both tanks sit comfortably on most stirring plates
Open design, rigid cassettes maximise gel-to-membrane compression for efficient current transfer; colour-coded to prevent polarity reversal