clinical chemistry assay kit / citric acid / urine / enzymatic
CC00150 LTA

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Characteristics

  • Applications:

    for clinical chemistry

  • Tested parameter:

    citric acid

  • Sample type:

    urine

  • Analysis mode:

    enzymatic, colorimetric

Description

Clinical Chemistry Specialties
/ Citric Acid in Urine
Enzymatic colorimetric determination of citric acid in urine.
The Citric Acid (citrate) is changed in oxalacetate and acetate by CL (Citrate lyase).
In presence of Malate-dehydrogenase (MDH) and Lactate-dehydrogenase (LDH),
the oxalacetate and pyruvate (decarboxilated product of oxalacetate), are trasformed in
L-Malate and L-Lactate, giving oxidization of NADH in NAD+.
The formation of NAD+ causes a diminution of absorbance at 340 n