Medical research assay kit Dual-Glo®

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Renilla luciferase internal control provides more accurate results Assay cells directly in growth medium for both reporters Extended half-life of 2 hours for each reporter An Extended-Life Dual-Luciferase Assay with "Add-Mix-Measure" Convenience The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. The convenient "add-mix-measure" protocol generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false-positive and false-negative readings caused by nonspecific factors such as cytotoxicity. In the Dual-Glo® Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results. The system is optimized for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media. The Dual-Glo® Luciferase Assay System provides high Z´-factors for cell-based, high-throughput screening applications. Assay Advantages Increased Precision and Accuracy. Assay normalization using an internal control co-reporter minimizes the effects of variations in cell number and health, transfection efficiency and nonspecific cellular responses. No centrifugation or pre-lysis steps required. Reagents are added directly to growth medium for both reporters.