Proteinase K reagent RP02503
for researchlyophilizedpowder

proteinase K reagent
proteinase K reagent
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Characteristics

Type
proteinase K
Applications
for research
Format
lyophilized, powder
Tested parameter
calcium, genomic DNA, urea
Storage temperature

Min.: -20 °C
(-4 °F)

Max.: 8 °C
(46 °F)

Purity

95 %

Description

No DNase contamination detected by digestion of λ DNA at 37 ℃ for 6 hours. No RNases contamination detected by digestion of λ DNA at 25℃ for 16 hours. Host genomic DNA/RNA is no residual detected by PCR. Bio-Activity - Measured by its digestion activity. The activity is >30U/mg. One unit will digest Casein at 37°C (pH 7.5) per minute to produce equal absorbance as 1.0 µmol of L-tyrosine. Endotoxin - Please contact us for more information. Formulation - Lyophilized powder is recommended to dissolve in in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, 5mM CaCl2, pH 7.2-7.4. Species - Tritirachium album Background Proteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachinm album Limber. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0 . Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction . Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA. Product information Synonym - Proteinase K Expressed Host - Pichia pastoris Tag - Tag Free

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