Antibody reagent kit RM17576

diagnosticRNA Synthesisprotein

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. Immunogen - HK293 derived SARS-COV-2 Nucleocapsid Ser2-Ala419 Purirty - Affinity purification Ig Type - Rabbit IgG Endotoxin Level - < 1.0 EU/μg of the protein by LAL method. Formulation - Supplied as a 0.2um filtered solution in PBS with 0.1%Braveds MB-1, PH 7.4. CrossReactivity - Has cross-reactivity in ELISA with SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Cat# RP01281) No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein HCoV-229E Nucleoprotein protein