Monoclonal antibody A19524

for Western blotimmunohistochemistryfor immunofluorescence

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. Immunogen information Immunogen - Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_733821.1). Gene ID - 4000 Swiss prot - P02545 Calculated MW - 74kDa Observed MW - 68KDa/72KDa Applications Recommended dilution - WB 1:10000 - 1:600000 IHC-P 1:50 - 1:400 IF/ICC 1:50 - 1:200 IP 1:10000 - 1:120000 Storage buffer - Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thiomersal, 0.05% BSA, 50% glycerol, pH7.3. Application key - Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples - HeLa, A-549, NIH/3T3, PC-12 Cellular location - Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm Customer validation - (Caenorhabditis Elegans) WB(Mus musculus) IF(Homo sapiens) WB(Homo sapiens) Western Blot(Mus musculus)