Featured - Specification
Applications - Restriction Enzyme Digestion
Unit Definition - One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA (dam methylated) in 1 hour at 37°C in a total reaction volume of 50 µl.
Storage Conditions - 10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/ml BSA, 50% Glycerol pH 7.4 @ 25°C
Storage Temperature - -20°C
Reaction Conditions - 1X Buffer CutS, Incubate at 37°C.
Reaction Buffer or Mix - 1X Buffer CutS:
50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°C
Heat Inactivation - 80°C for 20 min
Methylation Sensitivity -
dam methylation - Not Sensitive
dcm methylation - Not Sensitive
CpG Methylation - Blocked by Overlapping