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Enzyme reagent RK21109
for microbiology

enzyme reagent
enzyme reagent
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Characteristics

Type
enzyme
Applications
for microbiology

Description

Featured - Specification Applications - Restriction Enzyme Digestion Unit Definition - One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA (dam methylated) in 1 hour at 37°C in a total reaction volume of 50 µl. Storage Conditions - 10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/ml BSA, 50% Glycerol pH 7.4 @ 25°C Storage Temperature - -20°C Reaction Conditions - 1X Buffer CutS, Incubate at 37°C. Reaction Buffer or Mix - 1X Buffer CutS: 50 mM Potassium Acetate, 20 mM Tris-acetate, 10 mM Magnesium Acetate, 100 μg/ml BSA, pH 7.9 @ 25°C Heat Inactivation - 80°C for 20 min Methylation Sensitivity - dam methylation - Not Sensitive dcm methylation - Not Sensitive CpG Methylation - Blocked by Overlapping

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