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Enzyme reagent RK21115
for microbiology

enzyme reagent
enzyme reagent
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Characteristics

Type
enzyme
Applications
for microbiology

Description

Featured - Specification Applications - Restriction Enzyme Digestion Unit Definition - One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μL. Storage Conditions - 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C Storage Temperature - -20°C Reaction Conditions - 1X Buffer CutC. Incubate at 37°C. Reaction Buffer or Mix - 1X Buffer CutC: 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 100 μg/ml BSA, pH 7.9 @ 25°C Heat Inactivation - 80°C for 20 min Methylation Sensitivity - dam methylation - Not Sensitive dcm methylation - Not Sensitive CpG Methylation - Not Sensitive

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