Entrans 2X qPCR Probe Master Mix is a special reagent for qPCR reactions based on the primer-probe detection. When a probe is bound to the template DNA strand, the 5'-3 'exonuclease activity of Taq enzyme can be used to hydrolyze the probe, thereby releasing fluorescent signal. This product uses chemically modified hot-start Taq enzyme for amplification. Accurate quantification, high amplification efficiency, good repeatability and wide credibility. Entrans 2X qPCR Probe Master Mix is a 2X concentration master mix, which contains all the components required for qPCR except primers, probes and templates.