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DNA polymerase reagent kit PR1000-TP, PR1001-TP Seriesfor PCRhot start

DNA polymerase reagent kit
DNA polymerase reagent kit
DNA polymerase reagent kit
DNA polymerase reagent kit
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Characteristics

Type
DNA polymerase
Applications
for PCR
Other characteristics
hot start

Description

Accuris Taq Plus DNA Polymerase, an optimized blend of our Hot Start Taq and a proofreading polymerase, provides up to 5x better fidelity than standard Taq and increased enzyme activity. Higher fidelity for long PCR amplicons, up to 35kb. Ideal for problematic templates. Better sensitivity and higher activity for low copy and long PCR Enzyme of choice for TA cloning. Use our convenient PCR Selector Tool to determine the correct Accuris PCR Reagent for your applications. Accuris Taq Plus, an optimized blend of our Hot Start Taq and a proofreading polymerase, provides 5 times better fidelity than wild-type Taq and increased enzyme activity. It is the perfect choice for GC and AT rich templates, low copy number and long PCR. The 3′-5′ exonuclease activity of the proofreading enzyme produces fragments suitable for TA cloning. Accuris Taq Plus is provided with a 5X buffer (dNTPs not included) or in a convenient one tube 2X Master Mix. Accuris Taq Plus demonstrates high processivity even in the presence of crude lysates. The gel image shows amplification of an 850bp fragment of human chromosome with stretches of problematic AT and GC rich content. The first three lanes show the PCR product of amplification of 50ng, 5ng and 2.5ng amplified in the presence of crude lysate. The three right most lanes show the same amplification without the crude lysate.
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