The Accuris 1 Hour Mammalian Genotyping Kit is quick and easy – add sample to the proprietary buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes.
DNA extraction and amplification in 1 hour.
Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues.
Single tube – no organic solvents or clean up procedures.
Enhanced Accuris Hot Start Polymerase included.
Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.
The Accuris Mammalian Genotyping Kit is quick and easy to use – add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR Accuris Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed – just add sample and primers.
Lanes 1, 2 and 3 compare the Accuris 1 Hour Mammalian
Genotyping Kit with traditional methods for 3mm mouse
tail snips. Lane 1 - Accuris Kit, 1 hour workflow. Lanes 2
and 3 - traditional Proteinase K digestion and PCR with Taq
polymerase mix, overnight incubation. Lanes 4-7 - Replicate
PCR amplification from mouse ear tissue (2.5mg) using the
Accuris kit showing reproducibility.