Optimase® Polymerase is a thermostable DNA polymerase with an efficient 3′ to 5′ proof-reading exonuclease activity developed for unprecedented PCR performance in single nucleotide polymorphism (SNP) and mutation discovery studies. Initially developed for denaturing high performance liquid chromatography (DHPLC) research1-4, Optimase® Polymerase is the enzyme of choice for other applications requiring high fidelity PCR amplification.
• SNP and mutation discovery and scoring with various techniques such as SURVEYOR® Nuclease genome scanning, DHPLC, SSCP, DGGE and others
• High fidelity cloning
High fidelity proofreading polymerase. It has a 3’-5’ exonuclease activity.
Enhanced Optimase® Polymerase Buffer System
Free online support and access to the automated Optimase® ProtocolWriter™ for PCR protocol design and Optimase® MasterMix Calculator™ found at www.MutationDiscovery.com™
Offers superior PCR fidelity over other proofreading polymerases1-3.
Enhances the life of the DNASep® Cartridge in DHPLC experiments; does not interfere with other downstream applications, and increases amplification robustness and fidelity
Enables simple and automated generation of PCR protocols; thus minimizing PCR optimization.
Muhr, D., Wagner, T. and Oefner, P. 2002. Polymerase chain reaction fidelity and denaturing high-performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci, 782:105-110. Abstract Library Entry.
Nickerson, M. 2003. “Influence of PCR on DHPLC Polymorphism Characterization.” In Hecker, K. (Editor): Genetic Variance Detection: The Nuts&Bolts of DHPLC in Genomics. 2003, DNA Press.