Solution reagent RS-2100

cell imaging

MatriClone is a new matrix designed to support iPSC growth in single seeding workflows while maintaining pluripotency. MatriClone is optimized for ‘in-solution’ seeding and optically suited for automated imaging. When used as part of the VIPS seeding protocol, MatriClone offers high levels of single cell survival and subsequent outgrowth, all in a simple, automated workflow which avoids the need for coating plates. MatriClone is a defined matrix consisting of a recombinant peptide and is animal component-free. Colony outgrowth image captured on the VIPS at day 14 illustrating the enhanced number of colonies from VIPS seeded plates compared with limiting dilutions (LD). A New Standard For iPSC Support Why the need for change? Existing methodologies can include the likes of MatriGel, a chemically undefined mixture secreted from mouse sarcoma cells which must be manually coated onto plates. Often used with limiting dilution, a low efficiency statistical approach to single cell seeding, this approach is time-consuming and lacks in evidence of clonality. Designed For Use With Automated Imaging MatriClone offers ideal optical quality to contribute to high-quality data when imaged by automated imaging platforms. This makes MatriClone ideally suited to workflows using post-seeding technologies including the Cell Metric® whole well imager. Maintenance of Phenotype, Karyotype and Pluripotency Importantly, clones generated using the VIPS + MatriClone workflow have been shown by immunocytochemistry and karyotyping to maintain pluripotency after at least 10 passages and have retained their ability to successfully differentiate.