Medical research detection kit FS-HMM-0003

Sperm Vitalityfertilitypharmacology

Product Name Sperm Vitality Detection Kit (Eosin-Nigrosin Staining)

Short Description Distinguishes live from dead sperm using eosin-nigrosin staining based on membrane integrity; includes ready-to-use reagents and a clear protocol for brightfield microscopy viability assessment.

Product Introduction Sperm vitality (percentage of live sperm) complements motility measurement in routine semen analysis. The eosin-nigrosin technique differentiates cells by membrane integrity: non-viable sperm with compromised membranes take up eosin and appear red, while viable sperm exclude the stain and remain unstained against a dark nigrosin background. This kit supplies pre-prepared, quality-controlled staining solutions and a validated protocol to support consistent viability counts.

Product Application

• Routine semen analysis in human and veterinary andrology laboratories.
• Post-thaw viability assessment after sperm cryopreservation.
• Research on male infertility, toxicology, pharmacology or environmental impacts on sperm viability.
• Quality control in animal breeding and artificial insemination centers.

Product Advantages

• Standardized method: Technique recommended by WHO for semen analysis.
• Clear differentiation between live (unstained) and dead (red-stained) sperm for straightforward counting under brightfield microscopy.
• Ready-to-use, pre-mixed reagents reduce preparation variability and save handling time.
• Stable formulation with defined storage conditions to maintain performance over the stated shelf life.

Technical Specifications

• Product Name: Sperm Vitality Detection Kit (Eosin-Nigrosin Staining)
• Catalog No.: FS-HMM-0003
• Description: Differentiates live and dead sperm via eosin-nigrosin staining for viability assessment.
• Regulatory/Registration: FDA
• Storage: 2-8°C
• Shelf Life: 12 months unopened; 30 days after opening
• Certification: FDA
• Pack Size: 20 tests/kit