Solution reagent kit APO-Easy®

for DNA extractionNGSfor genotyping

The APOE gene on chromosome 19, responsible for coding apolipo-protein E, is genetically linked to both common late-onset familial and sporadic forms of AD. The human APOE gene comprises three alleles: -ε2, -ε3, and -ε4 distributed in the population at 7%, 79%, and 14%, respectively. Having one or two copies of the ε4 allele in APOE gene is statistically associated with an increased risk of developing AD. Approximately 65% of AD patients in the Caucasian population carry the APOE ε4 allele. ACCURACY RATE Exhibiting outstanding analytical performance with perfect reproducibility and repeatability. HOURS to complete the entire process REDUCTION IN MASTER MIX VOLUME yet the reliability is maintained demonstrating its robustness LIMIT OF DETECTION ensuring sensitivity in detecting target genetic material. Streamlined process Extract Genomic DNA (gDNA) extraction from whole human blood, saliva and buccal swab collected into PAXgene Blood DNA tubes and quantification. Run qPCR amplification and fluorogenic detection of APOE mutations using QS™ 5 Dx RT-PCR System and CFX Opus 96 Dx RT-PCR System Analyze Sample result analysis and interpretation. qPCR genotyping AD genotyping involves identifying genetic variations, using molecular biology techniques such as PCR, Sanger sequencing, and Next-Generation Sequencing (NGS). Method selection depends on study goals, scale, cost, and desired resolution. Key assay parameters OPERATING TIME The procedure comprises two phases: 30 minutes for plate preparation and 1 hour and 29 minutes for the qPCR cycle. ASSAY VALIDATION Validated on the QuantStudio 5 Dx Real-Time PCR system