Fluorescence microscope light source ILE

laser

Andor's laser engine portfolio offers exceptional quality within highly flexible devices. Each laser engine offers up to 10 lines from the UV to the NIR aiming to meet users' current and future needs. They accommodate a wide range of applications in life sciences from super resolution microscopy to long-term, gentle live imaging, TIRF and photostimulation. HLE - High Power Laser Engine – accommodates the widest range of applications from high-power for SMLM to low-power, long-term live imaging. NEW VLE – Versatile Laser Engine – Delivers high-quality illumination for live or fixed cell imaging. If higher laser power is needed, it is field upgradeable to HLE. Up to 10 laser lines Wavelength range 405-785 nm 150 - 2500 mW range 3 output ports SMLM - Super-resolution (DNA –PAINT) In DNA-PAINT - Point Accumulation In Nanoscale Topography - the sample is labelled with antibodies conjugated to short, single strands of DNA called docking strands (non fluorescent). The imager strand (attached to the fluorochrome) is in solution and binds stochastically to the docking strand creating the “blinking events”. HLE provide intense laser illumination to highlight binding events for high-precision localization. HLE in combination with B-TIRF 6-20 nm resolution can be achieved SMLM - Super-resolution (dSTORM) dSTORM - uses small organic dyes (such as AF647, Atto488) that can have 3 excitation sates. High laser powers are needed to achieve the dark state. The patented Borealis® optical configuration allows illumination zoom (iZoom) in the Dragonfly 600 series. iZoom enables power density multiplication in reduced fields of illumination.