rapid Helicobacter pylori test / for gastrointestinal infections / ammonia / breath
for gastrointestinal infections
Helicobacter pylori, ammonia
HELIC ABT with indicator tube is designed for primary noninvasive diagnosis H.pylori infection, as well as for monitoring the status of H.pylori during treatment and for effectiveness checks of therapy which already had been conducted.
Description of the method
HELIC Ammonia Breath Test was designed to exclude pain and discomfort during the diagnostic procedure. HELIC ABT is applied by gastroenterologists, general practitioners. No samples need to be sent to a central laboratory.
This is the biochemical method is based on produces a large amount of urease. H.pylori produces a large amount of urease, which degrades urea to ammonia (NH4+). HELIC ABT uses kinetic registration of ammonia concentration in patient’s breath air before and after a portion of unlabelled urea 12C is taken. The value of ammonia level is expressed in color change of indicator composition inside the tube.
Test procedure is very easy. Analysis consists of 2 parts:
Part I. Detection of basal level value.Patient to take the tube into his/her mouth. Aspirate the breath air during the period indicated at the label on the pump (6 or 7 minutes).
Part II. Detection of load level value after a portion of urea is taken. Ask the patient to take 0,5 g urea dissolved in 50 ml water. After he/she drinks turn the indicator tube the other side and start the second aspiration.
When test finished compare the basal level value with the load level value. Count the delta by distracting the basal level value from the load level value.If the delta exceeds the 3 mm, the test is positive; the delta is less than the 3 mm, the test is negative.