Magnetic bead-based reagent EmerTher®Ni-NTA

for protein analysisfor protein purificationprotein

EmerTher Ni-NTA Magnetic Beads are nano-superparamagnetic beads coupled with nickel-charged nitrilotriacetic acid (Ni-NTA). With a fast magnetic response rate, high protein binding capacity and low non-specific binding, EmerTher Ni-NTA Magnetic Beads provide a rapid and efficient method to purify His-tagged fusion proteins from crude cell lysates. Product Features NTA has four chelation sites for nickel ions and thus binds nickel more tightly than other metal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel ion leaching and results in a greater binding capacity and higher purity of the extracted protein than those obtained using other metal-chelating purification systems. Can be re-used after bead regeneration Binding capacity: 10-60 µg His-tagged fusion proteins per mg beads