Solution reagent kit 20162400221

tissuecalibrationliquid

It is used to quantitatively detect the content of lipoprotein a in human serum in vitro. Lipoprotein A is now considered to be an independent risk factor for atherosclerosis. Increased levels are seen in ischemic heart disease, cerebrovascular disorders, peripheral atherosclerosis, diabetes, kidney disease, connective tissue disease, etc. The significance of the reduction is unclear. 【Testing Principle】 Lipoprotein a in serum samples binds to anti-human lipoprotein a antibody bound to the surface of latex particles to produce an antigen-antibody reaction and agglutinate latex particles. The agglomeration of latex particles increases the turbidity of the reaction solution, which is measured by the change of absorbance at 700 nm. A calibration curve of absorbance for concentration can be established by measuring a series of calibration products. The concentration of lipoprotein a in samples can be determined by comparing with the calibration curve. 【Test Method】 1. Reagent preparation: liquid double reagent, no need to prepare direct use. 2. Test conditions: The test environment is not more than 95% at temperature (15-37)degrees Celsius and relative humidity. 3. Calibration procedures: 3.1 The matching calibrator is used. The calibrator is liquid and can be used directly. 3.2 A series of calibration products were prepared by diluting the calibration products with deionized water according to the table below. 3.3 The calibration curve is established in the non-linear mode (see the attached page for the specific mode of different instruments).