Fixative solution reagent 04-230828

decalcifying solutionfor hematologytissue

Product for the preparation of cyto-histological samples for optical microscopy. Decalcifying fixative for bone marrow biopsy in the study of hematologic diseases. The kit includes 2 reagents: fixative and decalcifier. - Zinc formalin is recommended for hematopoietic tissues; it contains formalin and zinc in a buffer solution. Concentration of zinc chloride has been chosen so that no zinc will precipitate on section during decalcifying process, provided that suggested procedure times are followed. - Decalcifier contains E.D.T.A. (ethylenediaminetetracetic acid) in acid buffer. Decalcifying process takes place through chelation in acid environment and preserves tissue morphology in a remarkably short time for histopathological routine. Method 1) Put bone marrow immediately after drawing in reagent A (fixative): leave to act 90 minutes. 2) Extract the fragment from solution A and put it in 70° ethanol for 15 minutes (the fragment can be left for an indefinite time) 3) Put the biopsy in reagent B (decalcifier) : leave to act 90 minutes. 4) Dehydrate through ascending alcohols, clear in xylene and embed in paraffin. ATTENTION: Suggested procedure times have been tested on cylinders of bone marrow biopsy 3 mm thick, taken from crista iliaca and sternum. Decalcification times should be prolonged for compact bone and larger fragments. We recommend to avoid fragments thicker than 5 mm. Storage Store the preparation at room temperature. Keep the containers tightly closed. Stability After the first opening, the product is reusable until the expiry date, if correctly stored. Disposal Hazardous preparation: observe all state