Buffer solution reagent MIELODEC
fixative solutiondecalcifying solutionfor histology

buffer solution reagent
buffer solution reagent
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Characteristics

Type
buffer solution, fixative solution, decalcifying solution
Applications
for histology
Format
liquid

Description

Fixative and decalcifier for bone marrow biopsy - Number of tests Volumetric ratio: specimen volume/ fixative volume 1:50 specimen volume /decalcifying solution volume 1:50 - Procedure time: 3 hours and 15 minutes - Product validity: 2 years - Storage temperature: 15-25 °C Expected aim Product for the preparation of cyto-histological samples for optical microscopy. Application Decalcifying fixative for bone marrow biopsy in the study of hematologic diseases. Principle The kit includes 2 reagents: fixative and decalcifier. - B5 fixative is recommended for hematopoietic tissues; it contains formalin and mercury in a buffer solution. Concentration of mercury chloride has been chosen so that no mercury will precipitate on section during decalcifying process, provided that suggested procedure times are followed. - Decalcifier contains E.D.T.A. (ethylenediaminetetracetic acid) in acid buffer. Decalcifying process takes place through chelation in acid environment and preserves tissue morphology in a remarkably short time for histopathological routine. Method 1) Put bone marrow immediately after drawing in reagent A (fixative): leave to act 90 minutes. 2) Extract the fragment from solution A and put it in 70° ethanol for 15 minutes (the fragment can be leftfor an indefinite time) 3) Put the biopsy in reagent B (decalcifier) : leave to act 90 minutes. 4) Dehydrate through ascending alcohols, clear in xylene and embed in paraffin ATTENTION: Suggested procedure times have been tested on cylinders of bone marrow biopsy 3 mm thick, taken from crista iliaca and sternum. Decalcification times should be prolonged for compact bone and larger fragments.

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