buffer solution reagent / for immunoanalysis / liquid
Primary container: amber bottle in polyethylene terephthalate (PET). Useful capacity 100 ml. PE cap. Tamper evident cap. The polyethylenterephthalate is a thermoplastic polymer of the polyester family. PET is an optimal oxygen, carbon dioxide and other gasses barrier. This material has an high resistance to ultraviolet radiation and an inertia toward the mainly chemical agents (solvents: xylene, limonene, liquid paraffines, alcohols, acids, bases etc.). It is biologically inert. It constitutes a good water and humidity barrier. It shows a great hardness and mechanical resistance. The bottle has an optimal grip. The absence of the handles reduces space for storage. The anti-dropping cap permits a precise and clean use. Secondary container: carton box.
Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol.
Expected aim Product for the preparation of cyto-histological samples for optical microscopy.
Application Citrate Buffer is used to recover the masked antigens of the tissue section fixed in formalin by microwave treatment.
Principle In order to perform immunostaining, the tissue specimens should be fixed in appropriate fixative. The purpose of such fixative is to preserve tissue structures and to immobilize antigens. However, this requires harsh treatment of the antigens. As a result, antigens undergo alteration of their secondary and tertiary structures. Because of changes in the protein containing epitopes or in neighboring proteins, antigenic sites may be masked.