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Toxoplasmosis test kit 3000-1214
IgGToxoplasma gondiiserum

toxoplasmosis test kit
toxoplasmosis test kit
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Characteristics

Applications
for toxoplasmosis
Tested parameter
IgG
Micro-organism
Toxoplasma gondii
Sample type
serum, plasma
Analysis mode
ELISA

Description

Principlebioelisa TOXO IgG is an ELISA test for qualitative and quantitative detection of IgG antibodies to T. gondii inhuman serum or plasma. The test is performed by incubating diluted test specimen in T. gondii antigen-coatedwells. Antibodies to T. gondii, if present in the specimen, will combine with the antigens attached to the well. Thewell is then washed to remove residual test specimen, and enzyme-labelled antibodies to human IgG (conjugate)are added. The conjugate will bind to anti-T. gondii IgG that have combined with the antigens on the well during thefirst incubation. After another washing to eliminate unbound material an enzyme substrate solution containing achromogen is added. This solution will develop a blue colour if the sample contains anti-T. gondii IgG. The bluecolour changes to yellow after blocking the reaction with sulphuric acid. The intensity of the colour is proportional tothe amount of anti-T. gondii IgG in the test specimen. The concentration of antibodies in the sample can bedetermined by mean of a calibration curve

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