Contamination detection kit EZ-PCR™

Mycoplasmacellbiological

Highly-sensitive PCR assay designed with enhanced specificity to test for over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures, with a detection limit of 10 CFU/mL. Includes ready-to-use reagents to perform 20 reactions, each with an internal control. Product Overview: The EZ-PCR™ Mycoplasma Detection Kit is a highly-sensitive and -specific PCR assay designed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures with a detection limit of 10 CFU/mL. The kit contains a ready-to-use PCR reaction mix with highly-optimized mycoplasma-specific primers, a positive control, and an internal control, all for the simple and efficient discovery of mycoplasma contamination. Samples can be prepared in as little as 10 minutes and results can be easily obtained within just a few hours. Easy to use. A complete PCR reaction mix that contains primers, Taq polymerase, and dNTPs is included. Easy to run. Follow a simple protocol with ready-to-use reagents for as little as 10 minutes of preparation. Easy to interpret. Internal and positive controls are included to ensure efficient and accurate PCR interpretation. Avoid False Negatives - Internal Control Included The EZ-PCR™ Mycoplasma Detection Kit includes an internal amplification control, a plasmid containing a non-mycoplasma-specific DNA sequence to test for false negatives. This internal control is simultaneously amplified in the tube with cell culture samples, and should be used with all PCR samples, including the negative and positive controls. This combination rules out inhibition from biological material,