Exicycler™ 96 Real-Time Quantitative Thermal Block is a self-patented 96-well simultaneous detection technology using LT (Light Tunnel) technology that emits uniform excitation light,excitation light blocking method using polarized light, and 2-D sensor. And it is a real-time gene amplification instrument that minimized optical sensitivity and deviation between wells. The thermal block is designed in a low-profile 96-well format, minimizing the volume of the solution used in the PCR reaction and reducing the PCR reaction time.
Optical Patent Technology
Bioneer patented fluorescence detection method (Korean Patent No. 10-1089045, US Patent No. US 8427643) was applied to minimize noise caused by reflected light, thus improving sensitivity and accuracy.
Noise caused by reflected light: The fluorescent materials used in qPCR create emission when the absorbed excitation energy excites them then falls to the ground state. In conventional qPCR instruments, part of excited light irradiated from the sample is reflected from the surface of the plate and the instrument, which may enter the detector to be also mistakenly read as part of the light from the sample. As the excited light has much more luminance than the fluorescent light, reflected light also contain high luminance, making it unable to be completely removed by the fluorescent light and creating noise in the process, deteriorating sensitivity and accuracy. However, by using our patented technology, excitation and fluorescence have different polarity, allowing the reflect light unable to be blocked by fluorescent filter to be filtered once again through polarizing filter.