AccuPrep® Bacterial RNA Extraction Kit enables rapid and convenient extraction of RNA from gram-positive and gram-negative bacteria. Proteins and other contaminants are effectively removed through silica-based spin columns and optimized buffers. This process is ideal for safe and convenient extraction since no organic solvents are used. Using AccuPrep® Bacterial RNA Extraction Kit, you can extract stable RNA without degradation.
Features and Benefits
Rapid and convenient extraction of RNA from gram-positive and gram-negative bacteria.
Complete removal of cell components such as proteins, nucleases and contaminants, resulting in improved efficiency and reproducibility of RT reaction
Minimized bacterial RNA degradation during extraction process.
Harmless protocol with no organic solvents used.
Easy to use because ethanol is added to Washing buffer
• cDNA synthesis
• Quantative real-time RT-PCR
• Northern blot analysis
Column loading volume 800 μl
Max binding capacity ~ 120 μg
Elution volume 30 μl – 100 μl
Preparation time ~ 40 min