foodproof® Sample Preparation Kit II
The Sample Preparation Kit II is designed for demanding isolation and purification of bacterial DNA from enriched food samples, including special matrices with a high proportion of PCR inhibitors. The kit is suitable for the detection of Gram-positive bacteria, such as Listeria and Alicyclobacillus.
Cells are lysed by incubation in foodproof Sample Preparation Kit II lysis buffer and lysozyme.
Extracted DNA is digested in proteinase K to destroy endogenous nucleases and other proteins.
The DNA is applied to the included glass fiber filter and purified with the foodproof Sample Preparation Kit II wash buffer.
Purified DNA is then eluted using the foodproof Sample Preparation Kit II elution buffer and is ready to use.
Isolation of highly purified DNA, virtually free of other cellular components and PCR inhibitors
Suitable for matrices containing PCR inhibitors