Blood/Liquid Sample Total RNA Rapid Extraction Kit (Spin-column)
The RNzol LS procedure represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology. A specialized high-salt buffer system allows up to 100 μg of RNA longer than 200bp binding to the RNzol LS silica membrane. Biological samples are first lysed and homogenized in the presence of a highly denaturing guanidine-thiocyanate–containing buffer, which immediately inactivates RNases to ensure purification of intact RNA. Ethanol is added to provide appropriate binding conditions, and the lysate transferred to the spin-column, where the total RNA binds to the membrane and contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100 μl water.
This kit can be used to isolate RNA from whole blood, biological fluids, and other liquid samples.
In addition, Lysis Buffer RLS is a great storage solution for total RNA. Lysate (add Lysis Buffer RLS to sample based on the volume ratio 3:1 and vortex rigorously) can be stored up to 2 months at -20℃ and half year at-80℃; also the lysate can be stable up for 1 day at 4℃, and 1 week at -20℃ in transportation.
*Convenience, no need to lyse erythrocytes and separate leukocytes for isolation total RNA from whole blood.
*Stability, comparable RNA yield with high quality absorbing membrane.
*High-purity, specific membrane absorption and wash-elution system ensure to remove protein and other debris.