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Antibody reagent GST-Trap
for Western blotveterinary laboratoryfor biochemistry

antibody reagent
antibody reagent
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Characteristics

Type
antibody
Applications
for Western blot, veterinary laboratory, for biochemistry
Tested parameter
protein

Description

Novel Binding Protein for Glutathione S-transferase Studies Analyze GST fusion proteins – in a tube Fusion of proteins to Glutathione S-Transferase (GST) of Schistosoma japonicum is a common technique to increase solubility and expression level of a protein. This tag also serves the additional function of an affinity tag for GST-fusion proteins. Binding of GST was historically accomplished by its natural substrate Glutathione SH, but is now usually performed with conventional antibodies. GST-fusions are often used in protein-protein interaction studies and biochemical analysis. Both approaches require highly specific tools to isolate and detect GST-fusion proteins. The conventional approach usually yields heavy and/or light chain contaminants. Now, with the GST-Trap, it is possible to get a much cleaner result—completely free of these contaminants–in under 30 minutes. Save time, save effort, and get cleaner results! The secret to GST-Traps success are the use of super-high affinity Camelidae antibody fragments called Nanobodies. GST-Traps may be used for immuno-precipitation, immuno-purification, and immuno-pulldown experiments with up to 10-fold better purity and yield than that of classic mouse monoclonal antibodies. Compatible with a variety of source materials, Nanobody-Traps may be used with mammalian cells, tissues & organs, bacteria, yeast, and even plants. These reagents allow your GST-fusions to be perfect candidates for immunoprecipitations, Co-IP, mass spectroscopy, and enzyme activity measurements.

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