Gamma-glutamyl transferase test kit 00028996

for blood diseasescoagulationglutamine

Protein-glutamine gamma-glutamyltransferase A chain Transglutaminase A chain Factor XIII, FXIII, F XIII, Factor 13 Gene name : F13A1, F13A A colorimetric microassay for quantification of Factor XIII activity More Information Principe The F XIII-Covtest uses biotinylated F11 peptide (Biotin-pepF11) as the first substrate (amine-acceptor/acyl-donor) and an amine-donor/acyl-acceptor as a second substrate. In the presence of activated F XIII (suspected to be contained in the samples), the γ carboxamide of the glutaminyl residue of the biotin-pepF11 is incorporated into the amine substrate to form biotinylated isopetide bound. The quantity of biotin incorporated is related to the FXIII activity and is revealed by a peroxidase colorimetric reaction. Labelling HRP Activity cross reaction - Measurement of activated plasmatic Transglutaminase XIII (FXIIIa) with low or no interference with other TGs isoforms. Species All Sp. Assay range 0.01 mU/ml to 0.5 mU/ml Storage instructions DTT and SAv-HRP enzyme at -20°C. Other reagents at +4°C. Target Plasmatic transglutaminase Uniprot ID - P00488 Publication - - Hitomi K, Kitamura M, Alea MP, Ceylan I, Thomas V, El Alaoui S. A specific colorimetric assay for measuring transglutaminase 1 and factor XIII activities. Anal Biochem. 2009 Nov 15:394(2):281-3. Description Microtiter strips coated with amine substrate DTT EDTA (Negative Control) Reaction Buffer (Biotin-pepF11/CaCl2) Enzyme Tracer (SAv-HRP) Wash Buffer 10X Diluent Buffer 10X HRP Substrate Blocking Reagent