Automated sample preparation system AP16 IF BLOT ELITE

IFAfor blot transferdilution

AP16 IF BLOT ELITE is an innovative and fast tool for the automation of IFA and BLOT methods, it is equipped with a barcode reader for samples and a camera for reading the barcodes of slides and Blot strips. The instrument is capable of processing 16 IFA slides with 12 wells, performing sample dilution, sample and reagent dispensing, incubation times and slide well washing. Thanks to the Blot module and the camera, it is able to perform complete automation for Dot/Western Blot, starting from sample dispensing and dilution, reagent dispensing, strip incubation and washing up to image processing and evaluation of each strip; the software also provides for the creation of reports and their archiving. TECHNICAL SPECIFICATIONS Barcode Reader - Integrated for samples, controls, and reagents Processing Capacity - on average 192 wells per session with incubation times of 30 + 30 minutes Sample Capacity - 180 sample tubes Ø13mm or 135 Ø16mm tubes that can be used in combination Trays - 264 positions for dilutions; 16 IFA slides; 20 controls; 8 conjugates (7 in the case of Evans Blue) and 1 wash needle solution; 2 sample diluents Liquid Containers - 2 canisters with wash buffer, Wash canister, and Waste canister, all equipped with a level sensor Slide Washing - Wells are washed individually to avoid contamination Washing Modes - multi-well, continuous flow, or drop by drop for specific washing effectiveness Liquid Handling - 2 needles: one for dispensing and diluting, the second for aspirating from the wells Dilution Time - 20s per sample with 2 ml of wash Dispensing Time - 11s per sample with 2 ml of wash Dispensing - CV <0.1%, inaccuracy 1% at 100µl