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Research reagent kit Omni-C™
NGS

research reagent kit
research reagent kit
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Characteristics

Applications
for research, NGS

Description

Specifications Reactions 8 Validated Samples Mammalian cells and tissues Technique Hi-C Labeling Research Use Only Application(s) Chromatin conformation analysis, SNP and structural variant detection Product Description The Dovetail™ Omni-C™ Kit uses a sequence independent endonuclease for chromatin digestion prior to proximity ligation. The Omni-C™ Kit provides all the reagents necessary for performing the proximity ligation steps on mammalian samples prior to NGS library generation. The kit is compatible with the Dovetail™ Library Module for Illumina (Cat # 25004) and 3rd party standard pair-end library preparation kits for Illumina sequencing. The Omni-C Kit offers the following key benefits: Sequence-independent chromatin fragmentation enables genome-wide detection of chromatin contacts (up to 20% of the genome lacks coverage using restriction enzyme based Hi-C approaches) Even genome-wide coverage, similar to shotgun sequencing, enables SNP calling, chromosome phasing, and structural variant detection Lower sequencing burden to reach desired depth of coverage saves time and cost Omni-C Libraries Enable Genome Wide Resolution of Chromatin Interactions Due to the use of a sequence independent endonuclease for chromatin digestion, the Omni-C Kit offers all the characteristics of a Hi-C approach without the sequence bias inherent to restriction enzyme based Hi-C approaches. Compared to restriction enzyme-based Hi-C approaches, Omni-C provides the following benefits: Significant overlap with data generated using restriction enzyme based approaches but enriched in long-range cis reads
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