Immuno-Blot test kit ALLERG4YLINE®

food intoleranceIgG4serum

Pre-coated line strips for easy Handling Panels with various food combinations available Cornes as a complété kit with ail necessary reagents and standards required to run the assay manually Reliable quantitative results, in kll/L and classes, in combination with a separately available scanner/software bundle Reliable semi-quantitative results in kll/L and classes if read out by naked eye The kit and the assay protocol can be adopted to the majority of the laboratory standard automated and semi-automated line blot analyzers Food intolerances are a growing burden worldwide. To address this, DST GmbH has designed nitro-cellulose based line strips for detection of specific IgG4. The assay is reliable, fast and cost effective. Multiple food panels and -sizes are available. Features and Benefits Detection range of specific IgG4 starting from 0.35 kUA/L No cross reactions with other immunoglobulins Inter-assay precision: mean coefficient of variation <10% Comes with a quadrilingual step-by-step quick guide for carrying out the assay and evaluation sheet (German, English, Spanish and Portuguese) Test principle Food extracts of the dedicated panel are embedded in nitro-cellulose strip membranes. For testing, a diluted sample (serum or plasma) is added to the incubation tray. Specific IgG4 antibodies of the sample bind to the spotted antigens on the membrane during incubation, and non-bound components of serum or plasma are washed away after incubation. Subsequently, anti-human IgG4 antibodies coupled horseradish peroxidase is added and bind to the IgG4 antibodies of the sample and the standards. Then the unbound detection antibodies are washed away.