legionellosis rapid diagnostic test / Legionella pneumophila / throat / sputum
EliGene® Legionella pneumophila
EliGene® Legionella pneumophila UNI is intended for DNA diagnostics of pathogenic species Legionella pneumophila from isolated DNA sample.
Principle of the method
This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit for the detection of Legionella pneumophila and internal control primers and Molecular Beacons labeled probes (FAM and HEX) are used.
Legionella pneumophila is a thin, aerobic, gram-negative, non-encapsulated, aerobic bacillus of the genus Legionella containing more than 50 species. It is the best-characterized member of the genus and major causative agent of Legionnaires' disease, a severe form of acute pneumonia. L. pneumophila is an intracellular pathogen when the bacteria invades and replicates in alveolar macrophages and epithelial cells. Macrolides (azithromycin) or fluoroquinolones (moxifloxacin) are the standard treatment for L. pneumophila in humans. About 3 to 8% of all community-acquired pneumonias are caused by L. pneumophilla when the case-fatality rate for patients with legionellosis is between to 30% with elderly and immunocompromised patients at greater risk of death.