lung cancer test kit / for EGFR mutations / blood / DNA
for lung cancer
for EGFR mutations
EliGene® Lung NGS is intended for multiplex PCR of target regions of EGFR gene (exons 18, 19, 20, 21) allowing detection of polymorphisms included in the amplified regions.
Principle of the method
EliGene® NGS kits enable multiplex PCR amplification of all required target regions of the gene(s) of interest. The recommended amount of DNA for each multiplex PCR reaction is between 20 and 100 ng of DNA derived from formalin-fixed paraffin-embedded (FFPE) material or from blood. Next, the resulting amplicons are barcoded with MID sequences, and mixed per sample. This sample is quantified and sequenced using a NGS instrument Illumina for massively parallel sequencing (MPS) according to the manufacturer’s instructions. The resulting sequence reads are subsequently analyzed by comparison with the reference sequence of the targeted gene(s) to identify variant positions.