Specifications
1. Intended Use
HbA1c Elution Buffer A, when used in conjunction with GSH-60 Automatic HPLC HbA1c Analyzer, is intended for in vitro diagnostic use for the
quantitative determination of Hemoglobin A1c (HbA1c) in human whole blood.
2. Test Principle
High Performance Liquid Chromatography (HPLC) is applied. Based on the charge differences, this method separates HbF, HbA1c and total HbA1 by
using the cation exchange column and three types of elution buffer with different salt concentrations. The percentage of HbA1c peak in relation to the
total area is calculated as the HbA1c concentration (%).