DNA polymerase reagent

NGS

The product amplifies multiple nucleic acid sequences at the same time through multiple PCR amplification technology, which could achieve high-fidelity PCR amplification of 100,000 to 10,000 pounds. The logarithm of self-customized Panel primers can be between ten ,and ten thousand pairs. The sequence junction is connected to the amplified target nucleic acid by ligase. The libraries prepared can be used on Ion Torrent or Illumina sequencing platforms. High data uniformity Uniformity＞95％ High library quality in complex areas High GC, high mutation, high repetition and other complex areas are of good quality Low template start amount The starting template can be as low as 1ng Wide range of amplifying enrichment enzyme The primer pairs from 10 to 10,000 pairs can be effectively amplified to build the library Use Company T and Ardent BioMed NGS DNA Library PrepSet to build up the library of Ardent BioMed TargetDrugs Panel (138) and Epilepsy Panel (9937) respectively, and use Ion Personal Genome Machine (PGM) System for sequencing. Taking gDNA and FFPE as templates, use T Company and Ardent BioMed NGS DNA Library PrepSet respectively to construct the library of Ardent BioMed TargetDrugs Panel and Epilepsy Panel. The analysis results showes that: From 138 pairs of small Panel to 9937 pairs of large Panel, sequencing depth of more than 97% of Amplicon has exceeded 20% of average sequencing depth. Data uniformity of library preparation kit of Ardent BioMed in small Panel is consistent with that of T Company, and data uniformity of large Panel is better than that of T Company