Infection test kit BST-SARS-25

SARS-COV-2coronavirusnasopharyngeal

This kit is used for the qualitative detection of nucleic acid from new coronavirus in nasopharyngeal swabs, oropharyngeal (throat) swabs, anterior nasal swabs, mid-turbinate swabs, nasal washes and nasal aspirates from individuals who are suspected of COVID19 by their healthcare provider. The detection of ORF1ab and N genes of the new coronavirus can be used for auxiliary diagnosis and epidemiological monitoring of new coronavirus infection. This kit is designed for specific TaqMan probes and specific primers designed for the novel coronavirus (SARS-Cov-2) ORF1ab and N gene sequences. The PCR reaction solution contains 3 sets of specific primers and fluorescent probes for specific detection of targets, and an additional set of specific primers and fluorescent probes is used as the internal standard control of the kit for detecting endogenous housekeeping genes. The test principle is that the specific fluorescent probe is digested and degraded by the exonuclease activity of the Taq enzyme in the PCR reaction, so that the reporter fluorescent group and the quenched fluorescent group are separated, so that the fluorescence monitoring system can receive a fluorescent signal, and then Through the enrichment effect of PCR amplification, the fluorescence signal of the probe reaches a set threshold value-Ct value (Cycle threshold). In the case of no target amplicon, the reporter group of the probe is close to the quenching group.