As part of the Hologic Invader suite of assays, Invader Factor II is an in vitro diagnostic test that simply and accurately screens for one of the four most relevant mutations associated with thrombophilia: human factor II.1-5
These innovative Invader assays detect and genotype single-point mutations in isolated genomic DNA obtained from whole blood samples.
Based on our Invader Plus chemistry, the Invader assays involve PCR-based target amplification followed by signal generation. Signal generation is composed of primary and secondary reactions:
Primary reaction — specifically and accurately detects the presence or absence of a particular target sequence.
Secondary reaction — used for signal amplification and result readout.
All necessary probes and oligonucleotides needed for target amplification and signal generation are included in the Invader Factor II assay kit.