Rapid heartworm test

for ehrlichiosisfor Lyme diseasefor canines

The Ehrlichia – Lyme - Anaplasma - Heartworm Combo Test (TBD-4) is a test cassette to diagnose the presence of tick-borne diseases antibodies against Ehrlichia canis, Ehrlichia ewingii, Borrelia burgdorferi, Anaplasma phagocytophilum and Anaplasma platys , and Dirofilaria immitis in dog’s serum or plasma specimen. PRINCIPLE The Ehrlichia – Lyme - Anaplasma - Heartworm Combo Test is based on sandwich lateral flow immunochromatographic assay. REAGENTS AND MATERIALS - Test devices - Assay buffer for EHR-LYM-ANA (Ehrlichia, lyme, anaplasma) - Assay buffer for CHW (Heartworm) - Products Manual STORAGE AND STABILITY The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight. SPECIMEN PREPARATION AND STORAGE 1. Specimen should be obtained and treated as below. - Serum or plasma: collect the whole blood from the patient dog, centrifuge it to get the plasma, or place the whole blood into a tube which contains anticoagulants to get serum. 2. All specimen should be tested immediately. If not for testing right now, they should be stored at 2-8℃. TEST PROCEDURE - Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay. - Take out the test card from the foil pouch and place it horizontally. INTERPRETATION OF RESULTS - Positive (+): The presence of both “C” line and zone “T” line, no matter T line is clear or vague. - Negative (-): Only clear C line appear. No T line. - Invalid: No colored line appears in C zone. No matter if T line appears.