Rapid cardiovascular disease test G6PD

hemoglobinbloodcassette

【INTENDED USE】 This test kit is used for qualitative detection of normal or deficient G6PD enzyme activity in whole blood samples. It is suitable for in vitro clinical auxiliary diagnosis of glucose-6phosphate dehydrogenase deficiency 【PRINCIPLE】 This product is based on the glucose-6-phosphate substrate colorimetric method. In the presence of NADP, glucose-6-phosphate is oxidized by G6PD enzyme in the sample, resulting in the production of 6-phosphogluconic acid lactone and NADPH. The light yellow nitrotetrazolium blue chloride (NBT) is reduced to insoluble blue-purple crystalline formazan in the reaction between NADPH and phenazine methosulfate (PMS). When G6PD enzyme activity is normal, insoluble blue-purple crystals are formed. Hemoglobin in the sample is removed by the filtration membrane, allowing the purple crystals to bind to the membrane, forming a blue-purple circular spot. However, when G6PD enzyme activity is abnormal or reduced, the blue-purple circular spot cannot be formed.