Proteinase K reagent kit GMAX MINI
for histologyfor PCRblood sample

proteinase K reagent kit
proteinase K reagent kit
proteinase K reagent kit
proteinase K reagent kit
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Characteristics

Type
proteinase K
Applications
blood sample, for histology, for PCR, for DNA extraction
Tested parameter
protein

Description

Sample Size: 50-200µl whole blood (fresh or frozen)/up to 25mg tissue/up to 25mg paraffin-embedded tissue/buccal swab/up to 15ml amniotic fluid/1×107cultured cells/25mg insects/10 hairs (including follicle cells) Expectant Yield: up to 50µg DNA Operation Time: 20min or less Elution Volume: 30-100µl Free sample available upon request The gMAX Genomic DNA Mini Kit has been optimized for genomic, mitochondrial, and viral DNA purification from whole blood (fresh and frozen) tissue, formalin-fixed paraffin-embedded tissue, cultured cells, buccal swab, amniotic fluid, hair, and insects in one convenient kit. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a wash buffer and the purified genomic DNA is eluted by a low salt elution buffer, TE, or water. The entire procedure can be completed in less than 30 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA is suitable for use in PCR or other enzymatic reactions. The quality of the gMAX Genomic DNA Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 µl of whole human blood. The purified DNA (5 µg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis. The gMax kit is one of the most versatile genomic DNA extraction kits on the market today. It has the ability to purify genomic DNA from a wide array of sample types with easy to use protocols and a very economical price point.

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