Dual signal optical mapping systems are suitable to record cardiac membrane potential and calcium imaging simultaneously. Our latest optical mapping system is equipped with the back Illuminated scientific CMOS cameras with 95% quantum efficiency (QE) and pixel size of 6.5 μm x 6.5 μm to 11 μm x 11 μm. It has a high speed and low noise acquisition with a dynamic range up to 50,000:1(94 dB). The optical axis can be constructed either vertically or horizontally. There are various I/O connectors for helping researchers to set up the communication system. OMap software also has an advanced intuitive user interface. A 8-channel TTL controller is designed for synchronizing devices such as camera, LED light controller, stimulator, ECG, MAP, pressure and temperature probe with the optical mapping system. The OMapRecord/Scope is an advanced data acquisition and analysis software which has an user-friendly intuitive interface and highly efficient data management.