NGS sequencing software Mix²

laboratoryanalysis

Precise transcript concentration estimates Accurate detection of differential expression Exceptional reproducibility across variable conditions Fast run-times and small memory footprint Mix² RNA-Seq Data Analysis Software A software tool for the accurate estimation of RNA concentration from RNA-Seq data. Model Fragment bias in RNA-Seq poses a serious challenge to the accurate quantification of gene isoforms. Mix² makes no assumptions about coverage bias but fits for each gene isoform a mixture model to the data (Fig. 1). Mix² can therefore, for instance, accurately represent the 5’ bias, as shown in Fig. 1 (a and b), whereas Cufflinks is restricted to the uniform distribution (Fig. 1c). A software tool for the accurate estimation of RNA concentration from RNA-Seq data.