Solution reagent kit LoopSeq™

sample preparationfor PCRfor DNA sequencing

This kit is a general purpose amplicon library prep kit researchers in any discipline with PCR fragments up to 3Kb can utilize to take advantage of Loop Genomics’ long-read technology. This kit features UMI that are added pre PCR for single-molecule counting eliminating PCR bias. It also features a multiplex workflow that pools 24 samples into a single tube with less than 3 hours of hands-on time. With example applications including but not limited to HLA, Pre-natal, Immune-repertoire, Oncology, CRISPR, Mutagenesis detection, Viral, Phage and Synthetic Biology this general purpose long-range PCR sequencing kit has a wide range of uses. Assay Time 8.5 hours Hands-On Time 2.5 hours Mechanism of Action Synthetic long read sequencing Multiplexing Allows for 24 samples to be processed in one tube Input Quantity 10 ng genomic DNA (2 ng/ul) Category any linear dsDNA input System Compatibility HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq, NovaSeq, MiniSeq, MiSeq Nucleic Acid Type DNA Method 2 x 150 Paired End (PE) sequencing Specialized Sample Types PCR products, genomes, metagenomes, synthetic DNA libraries, viruses Technology Assembly of a synthetic long reads form short reads Automation Capability Yes