This kit is a general purpose amplicon library prep kit researchers in any discipline with PCR fragments up to 3Kb can utilize to take advantage of Loop Genomics’ long-read technology. This kit features UMI that are added pre PCR for single-molecule counting eliminating PCR bias. It also features a multiplex workflow that pools 24 samples into a single tube with less than 3 hours of hands-on time.
With example applications including but not limited to HLA, Pre-natal, Immune-repertoire, Oncology, CRISPR, Mutagenesis detection, Viral, Phage and Synthetic Biology this general purpose long-range PCR sequencing kit has a wide range of uses.
Mechanism of Action
Synthetic long read sequencing
Allows for 24 samples to be processed in one tube
10 ng genomic DNA (2 ng/ul)
any linear dsDNA input
HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq, NovaSeq, MiniSeq, MiSeq
Nucleic Acid Type
2 x 150 Paired End (PE) sequencing
Specialized Sample Types
PCR products, genomes, metagenomes, synthetic DNA libraries, viruses
Assembly of a synthetic long reads form short reads