This kit selectively zooms into a bacterial population of microbiome samples and provides comprehensive profiling based on all 9 variable regions and barcode-based molecular counting. It also features a multiplex workflow that pools 24 samples into a single tube with less than 3 hours of hands-on time.
It’s the perfect kit for labs looking for better data from thier 16S sequencing runs. This kit can be used with fecal, oral, skin and other samples with high background non-bacterial DNA. This kit now works with for normal and low biomass samples allowing users to mix and match different sample types in one kit. Only 2-200 picograms of starting DNA are required for low input samples. Compatible with all Illumina instruments.*
*HiSeqs, NextSeqs, and NovaSeqs are recommended for per base cost benefits.
Mechanism of Action
Synthetic long read sequencing
Allows for 24 samples to be processed in one tube
10 ng genomic DNA (2 ng/ul)
HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq, NovaSeq, MiniSeq, MiSeq
Nucleic Acid Type
2 x 150 Paired End (PE) sequencing
Specialized Sample Types
Assembly of a synthetic long reads form short reads