This kit provides a comprehensive survey and accurate relative abundance of every bacteria, eukaryote, and archae in microbiome samples. Genetic profiling is based on all 9 variable regions and relative abundance using barcode based molecular counting. It also features a multiplex workflow that pools 24 samples into a single tube with less than 3 hours of hands-on time.
It’s the perfect kit for labs looking to profile every microorganism in their sample. This kit now works with for normal and low biomass samples allowing users to mix and match different sample types in one kit. Only 2-200 picograms of starting DNA are required for low input samples. Compatible with all Illumina instruments.*Compatible with all Illumina instruments.*
*HiSeqs, NextSeqs, and NovaSeqs are recommended for per base cost benefits.
Assay Time
8.5 hours
Hands-On Time
2.5 hours
Mechanism of Action
Synthetic long read sequencing
Multiplexing
Allows for 24 samples to be processed in one tube
Input Quantity
10 ng genomic DNA (2 ng/ul)
Species Category
Prokaryotes, Eukaryotes, Archae
System Compatibility
HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq, NovaSeq, MiniSeq, MiSeq
Nucleic Acid Type
DNA
Method
2 x 150 Paired End (PE) sequencing
Specialized Sample Types
Microbial sample
Technology
Assembly of a synthetic long reads form short reads