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DNA sequencing reagent kit LoopSeq™ 16S & 18S Read
for molecular biologygeneticbacteria

DNA sequencing reagent kit
DNA sequencing reagent kit
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Characteristics

Applications
for DNA sequencing, for molecular biology
Tested parameter
genetic
Micro-organism
bacteria

Description

This kit provides a comprehensive survey and accurate relative abundance of every bacteria, eukaryote, and archae in microbiome samples. Genetic profiling is based on all 9 variable regions and relative abundance using barcode based molecular counting. It also features a multiplex workflow that pools 24 samples into a single tube with less than 3 hours of hands-on time. We use the same technology for our 16S & 16S/18S Read Cloud Kits, we just sequence less, producing a less dense could of short reads. With the same number of barcodes as the standard kit, you'll still see even low-abundance molecules. However, with fewer reads/barcode, not every molecule has enough reads to make a full-length contig. It’s the perfect kit for labs looking to profile every microorganism in their sample. Compatible with all Illumina instruments.* *HiSeqs, NextSeqs, and NovaSeqs are recommended for per base cost benefits. Assay Time 8.5 hours Hands-On Time 2.5 hours Mechanism of Action Synthetic long read sequencing Multiplexing Allows for 24 samples to be processed in one tube Input Quantity 10 ng genomic DNA (2 ng/ul) Species Category Prokaryotes, Eukaryotes, Archae System Compatibility HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq, NovaSeq, MiniSeq, MiSeq Nucleic Acid Type DNA Method 2 x 150 Paired End (PE) sequencing Specialized Sample Types Microbial sample Technology Assembly of a synthetic long reads form short reads Automation Capability Yes

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.