Clinical Chemistry Specialties
/ Citric Acid in Urine
Enzymatic colorimetric determination of citric acid in urine.
The Citric Acid (citrate) is changed in oxalacetate and acetate by CL (Citrate lyase).
In presence of Malate-dehydrogenase (MDH) and Lactate-dehydrogenase (LDH),
the oxalacetate and pyruvate (decarboxilated product of oxalacetate), are trasformed in
L-Malate and L-Lactate, giving oxidization of NADH in NAD+.
The formation of NAD+ causes a diminution of absorbance at 340 n