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Magnetic bead-based reagent kit MF-HIS-3000 series
for protein purificationclinicalliquid

magnetic bead-based reagent kit
magnetic bead-based reagent kit
magnetic bead-based reagent kit
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Characteristics

Type
magnetic bead-based
Applications
clinical, for protein purification
Format
liquid
Tested parameter
protein, iron
Storage temperature

Min.: 2 °C
(36 °F)

Max.: 8 °C
(46 °F)

Description

-Easy to use -Fast operation without centrifugation -Good magnetism and high hydrophilic -Small scale (~μg) to large scale (~mg) protein purification Description Qbeads-NTA-Ni is designed for rapid purification of 6xHis-tagged proteins. Qbeads-NTA-Ni has nitrilo- triacetic acid (NTA) groups with charged nickel covalently bind to surface dextran of Qbeads. Due to the high affinity, Qbeads-NTA-Ni can be used for capturing 6xHis-tagged proteins. Bound 6xHis-tagged proteins can be temporarily immobilized under magnetic attraction, so the other parts in supernatant can be removed easily and efficiently. Bound proteins can be directly used in downstream applications or be eluted off the beads. The capacity of purified 6xHis-tagged proteins (~35kDa) captured by Qbeads-NTA-Ni is approximately 5 mg/mL. Specification: Core Iron Oxide (Fe3O4) Layer Dextran Surface protein NTA-Ni Mean Diameter of Particles ~1 μm Storage Buffer 20% ethanol Capacity 5 mg/mL
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